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JEH Quiz #6 (May 2010)

Featured Article:
“Rapid Detection of Enterococci in Marine Beach Water by Immunomagnetic Capture and Bioluminescence and Its Comparison with Conventional Methods ”

Available to NEHA members only, the JEH Quiz, offered six times per calendar year through the Journal of Environmental Health, is a convenient tool for self-assessment and an easily accessible means to accumulate continuing-education (CE) credits toward maintaining your NEHA credentials.

It's a simple process...

Read the featured article in the Journal.
Select the correct answer to each JEH Quiz question and click on the Submit button at the bottom of the page.
One CE credit will be applied to your account with an effective date of May 1, 2010 (first day of issue).
Check your continuing education account online.

Quiz deadline: August 1, 2010

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1.	E. coli and enterococci are considered to have higher correlation than fecal coliforms with outbreaks of swimming-associated gastroenteritis.
	a.	True.
	b.	False.
2.	The main sources of contamination of recreational beach waters include
	a.	sewer overflow.
	b.	failed household treatment systems.
	c.	agricultural runoff.
	d.	all of the above.
	e.	a and b only.
3.	For recreational marine waters, the U.S. EPA water quality standard requires
	a.	that no individual sample exceed 35 enterococci per 100 ml.
	b.	that the geometric mean of 5 samples spaced over 30 days not exceed 104 colony-forming units per 100 ml.
	c.	that the geometric mean of 5 samples spaced over 30 days not exceed 35 enterococci per 100 ml.
	d.	that the geometric mean of 30 samples spaced over 30 days not exceed 35 enterococci per 100 ml.
4.	Traditional detection methods have the following drawback(s):
	a.	they are not as sensitive as the immunomagnetic separation/adenosine triphosphate (IMS-ATP) method.
	b.	they are not as accurate as the IMS-ATP method.
	c.	they take 1 to 2 days to provide results.
	d.	all of the above.
	e.	a and b only.
5.	The IMS-ATP method has the following advantage(s):
	a.	it gives results within 30 minutes.
	b.	it counts only viable bacteria.
	c.	it yields values that are higher than those produced traditional methods, thus better ensuring the safety of bathers.
	d.	all of the above.
	e.	a and b only.
6.	Results of the authors’ study show that the IMS-ATP method is sensitive enough to detect enterococci counts below the U.S. EPA standard for individual samples.
	a.	True.
	b.	False.
7.	Results of the authors’ study show that the IMS-ATP method is sensitive enough to detect enterococci at counts that below the U.S. EPA standard for the geometric mean of samples taken over 30 days.
	a.	True.
	b.	False.
8.	Prefiltration was used in the study to
	a.	concentrate the bacteria.
	b.	remove large particles from the samples.
	c.	both a and b.
	d.	none of the above.
9.	For prefiltration of samples, a filter with 20-mm pores gives better results than one with 5-mm pores.
	a.	True.
	b.	False.
10.	For prefiltration of samples, a glass fiber filter gives better results than filters with either 5-mm or 20-mm pores.
	a.	True.
	b.	False.
	c.	Seal surfaces.
	d.	Emit a pleasing odor.
11.	Preparation of immunomagnetic beads involved the following steps:
	a.	disinfection with 0.1% sodium azide.
	b.	rinsing with sterile distilled water.
	c.	coating with enterococci antibodies.
	d.	all of the above.
12.	Exposing the solution to a magnetic field
	a.	separates the bacteria-bead complexes from the bacterial suspension by causing them to cling to the wall of the test tube.
	b.	separates the beads from the bacteria.
	c.	both a and b.
	d.	none of the above.